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Morphophysiological analysis of microplantlets of Cattleya labiata Linden and Cattleya eldorado Lindley (Orchidaceae) under the effect of paclobutrazol The Species Cattleya labiata Lindley and Cattleya eldorado Linden, native to the Atlantic Forest and Amazon, respectively, are under high risk of extinction in nature, therefore, necessary to develop efficient methods of spreading their seedlings, among which highlights micropropagation. The efficiency of in vitro cultivation of orchids is associated with culture media, and especially the growth regulators. The study aimed to evaluate the influence of paclobutrazol (PBZ) in the development of in vitro microplantlets. Seedlings from germination in vitro, with 1.0 ± 0.2 cm in length, were used in the study. In the first experiment, we used to control the MS culture medium free of plant growth regulators. In the treatments, MS medium was supplemented with four concentrations of PBZ 1.0, 2.0, 4.0 and 6.0 mg L, with naphthaleneacetic acid (NAA) 1.0 mg L associated with 6-benzylaminopurine (BAP) 1.0 mg L and with the combination of 4.0 mg L-1 PBZ with 1.0 mg.L NAA and 1.0 mg L BAP. The morphological evaluations, number of shoots, shoot length (CPA), longest root length (CMR) and leaf blade length (CLF) were performed at the beginning of the experiment and in subcultures every 40 days during 160 days. The experimental design was completely randomized in three-factor split-plot arrangement in time, with six replicates per treatment. A second experiment was performed using the technique of "pulse". It was used as control MS medium, and the treatments we used the same medium plus increasing concentrations of PBZ with different exposure times, as follows: T0 = control (MS), T1 = 1.0 mg L PBZ for 40 days, T2 = 10.0 mg L PBZ for 4 days and T3 = 100.0 mg L PBZ for 4 hours. Assessments were made CPA, CLF, CMR, number of roots, fresh and dry mass of shoots, fresh weight and dry weight of root, fresh weight and total dry matter, stomatal counting and characterization and evaluation of the anatomic root tissue. The experiment was conducted in a completely randomized a total of fourteen replicates per treatment. Treatment with 1.0 mg L of PBZ for 40 days promoted further development and vigor of the root system, increasing the thickness of the roots and may contribute to the acclimatization phase of microplantlets. Treatments with "pulse" significantly reduced the shoot microplantlets and It‘s not recommended to the micropropagation of C. labiata.

Análise morfofisiológica de microplântulas de Cattleya labiata Lindley e Cattleya eldorado Linden (Orchidaceae) sob efeito do paclobutrazol