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ZAFFALON, F. G. Capacitation-like changes in bovine semen after cryopreservation using extenders within egg yolk or soy lecithin. [Alteracoes semelhantes a capacitacao no semen bovino apos a criopreservacao utilizando diluidores a base de gema de ovo ou lecitina de soja]. 2009. 93 f. Dissertacao (Mestrado em Ciencias) – Faculdade de Medicina Veterinaria e Zootecnia, Universidade de Sao Paulo, Sao Paulo, 2009. The objective of this experiment was evaluate the effects of three diferent extenders in bovine semen cryopreservation about motility, sperm hiperactivation, acrosome reaction, capacitation, and sperm membrane lipid peroxidation. It was made six collection of semen samples each 15 days of 10 Nelore bulls. Semen samples in natura was verify and divided in three treatments: The first one was extended in egg yolk base (Botu-Bov Extender 1), second one was extended in soy lecithin base (Botu-Bov Extender 2) and the third was extended in AndroMed soy lecithin base too. After that, semen samples were submitted to freeze process. Semen evaluations after thawing were made with computer assisted sperm analysis (CASA) and flow citometry for acrosome reaction (PI/FITC-PSA/H33342), lipid peroxidation (PI/C11BODIPY/H33342) and sperm capacitation by plasma membrane stability (Merocianina 540/Yo-Pro1/H33342). Data obtained from experimental proceeding were submitted to variance analysis and LSD test to compare means with 5% of significance. Egg yolk base extender preserved better sperm motility, cell population with plasma membrane integrity and non-reacted acrosome in bovine semen after thawing than soy lecithin extenders. Cryopreserved sperm with egg yolk base extender displayed less subpopulation of hiperactivated cells, less destabilized plasma membrane and permitted a decrease of lipid peroxidation of membranes when it was compared with soy lecithin extender.

Alterações semelhantes à capacitação no sêmen bovino após a criopreservação utilizando diluidores a base de gema de ovo ou lecitina de soja