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FREITAS, G. D. Comparison between the growth of Colony Form Units of Staphylococcus spp. and Klebsiella pneumoniae and the sensibility of these microorganisms to the process of slow pasteurization. [Comparaç ã o entre o crescimento de Unidades Formadoras de Colô nias (UFC) de Staphylococcus spp. e Klebsiella pneumoniae e a sensibilidade destas cepas ao processo de pasteurizaç ã o lenta]. 2007. “88” f. Dissertaç ã o (Mestrado em Medicina Veterinária) – Faculdade de Medicina Veterinária e Zootecnia, Universidade de Sã o Paulo, Sã o Paulo, 2007. Introduction: The milk can be considered one of the most complete food for its high contents of protein and minerals, but also it is considered an excellent medium of culture for microorganisms. Objective: To identify the number of the Colony Forming Units of Staphylococcus spp. and Klebsiella pneumoniae, in integral sterile milk, at different temperatures (6C, 27C e 37C) and to describe a thermal curve death, when submitted to the process of slow pasteurization isolated or in association. Materials and methods: Evaluation by counting of the colony forming units and the behavior of the Staphylococcus spp. and Klebsiella pneumoniae, isolated and in association occurred in the cooling tank, submitted to temperatures that simulate the conditions of milk in cooling (6C), environmental condition (25C) and the optimal temperature for the growth of microorganisms (36C). Evaluation of the sensibility of Staphylococcus spp. and Klebsiella pneumoniae isolated and in association during the pasteurization process. Results: In Scale 1 of Mac Farland the average of the colony form units of the K. pneumoniae was greater than the colony of Staphylococcus spp. At 6C the microorganisms in the milk isolated or in association grew at the same speed. At 25C K. pneumoniae associated with Staphylococcus spp. grew more than when they were isolated. At 36C K. pneumoniae associated with Staphylococcus spp. grew more then when it was isolated and more than at 25C. So we can conclude that the slow pasteurization was effective, because it has reduced at least 90% of the colony form unity in the milk after 30 minutes at 65C. Conclusion: By raising the temperature up to 36C, a Klebsiella pneumoniae had a superior growth when compared with Staphylococcus spp.; the contamination of milk by Klebsiella pneumoniae (environmental contamination) influences the most the quality of the final product when compared to the contamination by Staphylococcus spp. from mastitis, we can also conclude that the interaction of microorganisms modify the death rate of them. New studies and researches are recommended to a deeper and better comprehension of this process.

Comparação entre o crescimento de Unidades Formadoras de Colônias (UFC) de Staphylococcus spp. e Klebsiella pneumoniae e a sensibilidade destas cepas ao processo de pasteurização lenta