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FERREIRA, J. B. Detection of Mycoplasma pulmonis and Mycoplasma arthritidis in the upper respiratory tract of rats and technicians by culture and polymerase chain reaction (PCR). [Detecção de Mycoplasma pulmonis e Mycoplasma arthritidis no trato respiratório superior de ratos e bioteristas por cultivo e reação em cadeia da polimerase (PCR)]. 2005. 94 f. Dissertação (Mestrado em Medicina Veterinária) – Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, 2005. Mycoplasma pulmonis and Mycoplasma arthritidis are species usually isolated from laboratory rats, causing losses and modifying results of research that use such animals. Mycoplasma pulmonis inhabit primary the respiratory tract, nasopharynx, and middle ear of murines causing the murine respiratory mycoplasmosis (MRM). In general, aerosols perform the transmission, which allows the spreading of this agent to all facilities departments and to technicians. Mycoplasma arthritidis can be recovered from the oropharynx of these animals, but its occurrence is rare. Mycoplasmas usually exhibit a rather strict host and tissue specificity, probably reflecting their nutritionally exacting nature and obligate parasitic mode of life. However, there are numerous examples of the presence of mycoplasmas in hosts and tissue different from their normal habitats. The aim of this study was to recovery Mycoplasma pulmonis and Mycoplasma arthritidis from laboratory rats and detection of these species by PCR in rats and technicians from distinct facilities. 144 (60%) samples of tracheal washed of rats were positives in culture to Mycoplasma pulmonis and 155 (64,58%) by specific PCR. Mycoplasma arthritidis was nor isolated, neither detected in any samples. M. pulmonis was detected in four (10%) of the samples collected from the technicians who did not maintain direct contact with rats, being one sample from facility 1 and three samples from facility 3. Regarding to technicians who keep direct contact, 24 (77,4%) were negatives on the two collects, 4 (12,9%) were positives after manipulating animals, 2 (6,5%) were positives in both collects, before and after treatment of animals and 1 (3,2%) was positive before cleaning the boxes. These results showed that people who work in facilities are exposed to this agent and can become a revervoirs of infection.

Detecção de Mycoplasma pulmonis e Mycoplasma arthritidis no trato respiratório superior de ratos e bioteristas por cultivo e reação em cadeia da polimerase (PCR)