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Metallothionein is an important protein for detoxifying heavy metal ions. Th is research was conducted to isolate and clone cDNA of gene encoding for metallothionein type 2 from Melastoma affi ne. Total RNA was isolated from young leaves. Total cDNA was synthesized from the total RNA by reverse transcription. Th e MaMt2 cDNA was successfully isolated by PCR technique. Th e MaMt2 cDNA was inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into Escherichia coli DH5α. DNA sequencing analysis showed that this cDNA is full length consisting of 246 pb encoding 81 amino acid residues. Th is cDNA is identical to mRNA of AtMt2 from Arabidopsis thaliana. It does not contain any restriction sites found in the cloning sites of pGEM-T Easy. Th e deduced protein of MaMT2 contains 14 cysteine residues distributed in the Cys-Cys, Cys-X-Cys, and Cys-X-X-Cys motifs.

ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE