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Th is experiment was conducted to evaluate the eff ect of phytase supplemented to the diet on phosphorus (P) digestibility and growth performance of the green catfi sh Hemibagrus nemurus. Five kinds of experimental diets were used in this experiment, namely diets A, B, C, D and E. Diet A, as a control, was supplemented with inorganic P, without phytase supplementation. Diet B, C, D and E were supplemented with 0, 20, 40 and 60 mg phytase/100 g soybean meal (SBM), respectively, without inorganic P supplementation. Fifteen fi sh with initial body weight of 6.9 + 0.2 g were stocked into each 60-l aquarium. Fish were fed on the diets for 60 days. Results indicated that P digestibility increased from 64.5% to 87.0% as phytase supplement increased from 0 mg in diet B to 60 mg phytase/100 g SBM in diet E. P digestibility in diet E was higher than that in diet A (77.6%). Th e daily growth rate and feed conversion ratio followed similar trend. P, Ca and Zn concentration in the whole body and bone of fi sh fed diet E were higher than the fi sh fed diet B, C and D, but were insignifi cant compared to the fi sh fed diet A. Nitrogen (N) and P loading by fi sh fed diet E were, respectively, 76% and 20% lower than those in fi sh fed the control diet. It is concluded that the inclusion of 60 mg phytase/100 g SBM in the diet of the green catfi sh could replace the utilization of inorganic P increase the digestibility of the diet thereby resulting in increased growth rate and reduced excretion of P and N into the waters.

EFFECT OF PHYTASE SUPPLEMENTATION IN SOYBEAN MEAL BASED DIET ON NUTRIENT DIGESTIBILITY AND GROWTH PERFORMANCE OF GREEN CATFISH (Hemibagrus nemurus)