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The DELFIA (LKB Wallac) AFP assay is a non-isotopic technique which utilizes a lanthanide metal (Europium) chelate label possessing highly fluorescent properties (Soini and Kojola [1]; Hemmila et al. [2]). The assay incorporates two monoclonal antibodies directed against two different immunogenic sites on the AFP molecule one of which is solid phase immobilized to the well of a microtitration strip.

Comparison of a time-resolved fluoroimmunoassay with a solid phase immunoradiometric assay the measurement of alphafetoprotein in amniotic fluid