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The use of changes in light-scattering properties as a means ofmonitoring the reaction between two species has been used for many years. In recent years interest has tended to concentrate on monitoring the turbidity of a reaction mixture. The reaction between polyvalent antigens and the appropriate antibodies may be monitored by the development of the turbidity as a result of immunoaggregate formation; this technique is used routinely for the quantitation of specific proteins. Reactions are monitored at 340 nm for short periods of time, generally less than 10 min and, as a consequence, results can be produced quickly.

Performance characteristics of a light-scattering immunoassay for thyroxine on a discretionary analyser