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The measurement of blood urea nitrogen (BUN) as serum or plasma urea is one of the most frequently ordered laboratory tests for evaluating renal functions. Conventional analysis of reactions of urea with diacetyl monoxime or urease with the Berthelot reaction [1] is rapidly giving way to the totally enzymatic reactions that are more convenient to perform and more easily adapted to modern instrumentation. However, the automated analysis ofthese reactions may yield falsely elevated BUN levels for some patients.

journal of automatic chemistry

Artifactually elevated BUN values on the Boehringer Mannheim-Hitachi 737 and 705 automated chemistry analysers and the development of a kinetic BUN method