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Liquid chromatography, and especially high pressure liquid chromatography, with the great resolving power of its microparticulate column materials are potentially the best techniques for the separation and determination of trace substances that have insufficient volatility for analysis by gas chromatography. Many of these trace substances contain a metal or a metalloid such as arsenic, selenium, tin or cobalt, that can be determined by atomic absorption spectrometry. These compounds may be essential for life [1], for example cobalamine, or pose a threat to organisms [2], for example arsenite. For environmental and health reasons, knowledge about the nature and concentrations of such substances in living and non-living matter is critical. These substances occur at mg/1 or txg/1 concentrations in complex matrices which will obscure chromatographic peaks of interest or make their identification difficult. To overcome these difficulties graphite furnace atomic absorption spectrometers [3-10] and plasma emission spectrometers [11 and 12] have been used as element-specific detectors for high pressure liquid chromatography in manual [4] and automated modes [3, 5, 8 and 12].

Element-specific detectors for high pressure liquid chromatography (HPLC): a computerized, automated HPLC-graphite furnace atomic absorption system