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Enzymatic-continuous-flow methods, essentially similar to those previously described ], are used by the authors to measure metabolites of carbohydrate metabolism in blood after protein precipitation and stabilization with perchloric acid. These techniques, combined with fluorimetry, allow up to 140 samples, for three metabolites, to be analysed each day. The precision obtained and the cost per sample compare very favourably with the tedious manual spectrophotometric methods.

journal of automatic chemistry

Computer acquisition and analysis of data in enzymatic-fluorimetric-continuous-flow methods for the measurement of glucose, lactate, pyruvate, alanine, glycerol and 3-hydroxybutyrate in human blood