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Simple and specific measurement of free fatty acid (FFA) levels in plasma and serum is of practical value to the clinical biochemist and, in the authors’ laboratory, in veterinary nutritional and metabolic studies. Most early methods, such as that of Duncombe [1], relied on the colorimetric determination of metal-FFA complexes in the organic phase of solvent extracted plasma. These techniques are relatively non-specific and, in addition, are extremely laborious and time-consuming. Recently, alternative assays for FFA, based on specific enzymic oxidation by acyl-coenzyme-A-synthetase (ACS) and acyl-coenzyme-A-oxidase (ACOD), have been developed (Shimizu et al. [2]). Hydrogen peroxide generated by these reactions can be quantitated colorimetrically (Mizuno et al. [3]; Shimizu et al. [4]; Matsubara et al. [5]) and is directly related to the FFA content of the test sample. One such procedure involving specific peroxidase (POD)-dependent quinone-dye formation is now available commercially. This article reports on the adaptation of the method .for routine automated determination ofFFA using an I.L. Multistat III microcentrifugal analyser.

Automated enzymatic determination of plasma free fatty acids by centrifugal analysis