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The bromocresol green (BCG) method was introduced by Rodkey [1] in 1965 for determination of serum and plasma albumin and it is still widely used--despite criticism of the available procedures I-2, 3, 4 and 5]. In 1976, Savory et al. [6] adopted a method for use in centrifugal analysis demonstrating good precision and accuracy. Although bromocresol purple has been suggested as an alternative dye !-7 and 8], this dye is not suitable for samples from some animal species nor for qualitycontrol sera (for example bovine sera). The lack of readily available specific antisera for some animal species, and the unsuitability of bromocresol purple, led the authors to develop an automated BCG method using the Cobas Bio (Roche) centrifugal analyser [9] and to compare this method with an immunoturbidimetric method for human albumin using the same instrument. Centrifugal analysers permit very rapid absorbance measurements to be made, thus avoiding the slower reactions resulting from the binding of certain alphaand betaglobulins with BCG. The over-estimation of plasma serum albumin at lower concentrations may be avoided by adjusting the assay conditions.

Plasma albumin determination on the Cobas Bio centrifugal analyser using bromocresol green