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Single-channel integrators have been used with gas-liquid chromatography (g.l.c.) equipment and amino-acid analysers for some years and are adequate for many g.l., applications. During amino-acid analysis with the conventional ninhydrin systemmost amino-acids give a blue product but others, notably proline and hydroxyproline, give a yellow product. These products are measured separately, so for manual calculation a two-pen, or three-pen, recorder has always been provided. Attempts to use a single-channel integrator for amino-acid analysis are inevitably unsuccessful. Measuring just the blue channel at 570 nm leads to gross inaccuracies for the small peaks of proline and hydroxyproline; combining the two colorimeter outputs leads to base-line problems, as does the use of an intermediate wavelength. There are now integrators available that enable the output from two or more channels to be integrated, but it is not always possible to combine the results from two channels. Amino-acid analysis results for proteins have been presented as residues per 1000 residues (or per 100 residues)--this method was first introduced as an aid to comparative studies and for this it is necessary to take data for proline, and hydroxyproline ifpresent, from the 440rim channel and combine it with data for all the other amino-acids from the 570rim channel. This paper presents a program that allows the stored data from two channels of a Trilab 3 integrator to be combined to give a full amino-acid analysis expressed in residues/1000 residues. The operator may elect to omit earlier blocks ofdata to reach an appropriate standard, and may also reassign peaks that were wrongly assigned in the automatic mode. With minimal modification the program could be adapted to combine data from two g.l., runs on a single sample and express results in any convenient form.

journal of automatic chemistry

Program in BASIC to combine the data from two channels of an integrator, and its use in the calculation of residues per 1000 residues amino-acid analyses