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The currently used SMAC glucose oxidase method is a modification of the procedure of Gochman and Schmitz ]. In this colorimetirc determination, the specificity of glucose oxidase is combined with a peroxidase indicator coupler, 3-methyl-2 benzothiazolinone hydrazone (MBTH) and dimethylaniline (DMA) to form a coloured indamine dye with a high molar absorption. However, as much as six different reagents have to be prepared by the user, some of them daily. Barham and Trinder [2] described in 1972 a method involving the use of 4-aminophenazone as a colour

Enzymic determination of glucose with SMAC: adaption of the dichlorophenol/ aminophenazone chromogen