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accepts signals from the unknown sample. There are facilities for inserting the standard value and the system automatically carries test reading out the calculation std. reading X standard value. There are also logarithmic facilities available and other linearising facilities have been considered but have not so far been needed. Work is in hand to replace this system and the control unit by a microprocessor. The entire fluid handling system together with the photometric unit was housed in a steel cabinet well insulated with polystyrene foam and thermostatted at 35 + 0.2. The system has been tested by estimating serum glucose with glucose oxidase in conjunction with a chemiluminescent reaction and total serum protein by a biuret method, (Weischelbaum [11 ]) but operating at 30.0 Chemiluminescence is a phenomenon that deserves more exploitation as a means of making kinetic observations, since the light level obtained is directly proportional to the rate of the reaction. In the method used, liberated hydrogen peroxide was allowed to react with an acridinium salt and the light emitted measured at 425 nm. This method was developed by one of us (DET) and will be fully described elsewhere. The reproducibility of the glucose method was excellent; 20 replicate aqueous samples of nominally 11.1 mmol/1 gave a mean of 11.09 mmol/1 and a coefficient of variation of 0.627o. This method is linear to within 070 up to 33.3 mmol/. 46 samples of plasma preserved with fluoride were measured, both by the chemiluminescent and hexokinase methods and gave a correlation coefficient of 0.996. The only disadvantage of the method is the relatively large volume (60 lal) of plasma needed for each test. For measurement of total protein, the machine was used in the absorbance mode with the arithmetic unit equipped with an analogue logarithmic converter. Assays of 20 replicates of bovine albumin 80 g/1 gave a mean of 80.33 and a coefficient of variation of 0.45070. It is interesting to note that the error in this analysis is very little more than that found in the sampler itself so that other measuring units make little contribution to the total error. The machine showed no departure from linearity up to 120 g/1.24 plasma samples with total protein ranging from 42 to 88 g/1 were assayed both by the emergency machine and the Vickers M300 and a coefficient of correlation of 0.980 was obtained. A number of other analytical methods have been tried under simulated conditions but have not yet been used on the machine. These include a fluorimetric rate method for glucose; a fluorimetric rate method for alkaline phosphatase and a urease urea method using conductivity to measure the rate of liberation of ammonia.

journal of automatic chemistry

The design of a microprocessor system for automatic signal averaging and percentage purity calculations coupled to a nuclear magnetic resonance spectrometer