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The binding of 3;3′-diethylthiacarbocyanine iodide (DTCC) with molecules of DNA was investigatedby different photochemical methods. Absorption spectra were investigated under various concentrationof DNA. Isosbestic points observed in these spectra are attributable to existence of two differentcomplexes DTCC with DNA. Equilibrium constants of the formation of complexes I and II were determined(K1≈106M−1, K2≈5×104M−1 accordingly). Differential absorption spectra of two photoisomers of DTCCwere obtained by flash photolysis method; it was found that the quantum yield of the short-lived photoisomerdecreases and the quantum yield of the second long-lived photoisomer builds up with the increase inthe DNA concentration. Kinetics of interaction between the triplet state of the dye and oxygen were investigatedunder various concentration of DNA. The growth of the quantum yield of fluorescence and intersystemcrossing was observed with the increase in the DNA concentration

Complex formation between 3;3´-diethylthiacarbocyanine iodide and DNA and its investigation in aqueous solution