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We have proposed the use of the fluorimetric method with 4-methylumbelliferylphosphate (4-MUP) specific substrate for the alkaline phosphatase determination in theneutrophil adhesion assay. We provide evidence that the endogenous neutrophil alkalinephosphatase (NAP) activity evaluation is reliable to quantify neutrophil adhesion at a wide range of cell numbers (104–106). The results obtained by fluorimetric NAP activity testcorrelate to the results of adherence evaluated using the MTT reduction assay. Thefluorimetric NAP activity test may be applied for resting as well as activated neutrophilswithout the risk of the activators interferences into the test. The alkaline phosphatase surveywith the use of 4-MUP substrate is recommended herein as a sensitive, repeatable, simple, andreliable method of the neutrophil adherence determination in vitro

Application of Intracellular Alkaline Phosphatase Activity Measurement in Detection of Neutrophil Adherence In Vitro