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In this study, Mn-doped ZnSe/ZnS core/shell quantum dots (CSQDs) were synthesized in aqueous solution using polyethylene glycol as a surface stabilizer and successfully applied in the detection of  Escherichia coli  O157:H7 and methicillin-resistant  Staphylococcus aureus  (MRSA) for the first time. The CSQDs were conjugated with anti- E. coli  antibody and anti-MRSA antibody via protein  A  supported by 1-ethyl-3-(-3-dimethylaminopropyl)carbodiimide hydrochloride for fluorescent labeling of the intact bacterial cells. The detection was performed for the bacterial strains cultivated in Luria-Bertani liquid medium. The obtained results indicate that  E. coli  O157:H7 and MRSA can be detected within 30 min at a high sensitivity of 10 1  CFU/mL. This labeling method based on the highly fluorescent CSQDs may have great potential for use in the food industry to check and prevent outbreaks of foodborne illness.

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Utilization of Mn-Doped ZnSe/ZnS Core/Shell Quantum Dots for Rapid Detection of <i>Escherichia coli</i> O157:H7 and Methicillin-Resistant <i>Staphylococcus aureus</i>

Utilization of Mn-Doped ZnSe/ZnS Core/Shell Quantum Dots for Rapid Detection of Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus