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The effect of divalent ions on the thermal stability of the double-stranded polynucleotide polyinosinic:polycytidylic acid (poly(I:C)) in a buffer solution (pH 7) was studied by UV absorption spectroscopy and thermal denaturation by measuring the absorbance at 260 nm. The concentration dependence of the polynucleotide melting temperature ( T  m ) in the presence of metal ions (2.5 × 10 −6 –10 −2  M) was obtained. It was found that the duplex thermal stability grew gradually upon an increase of Ca 2+  and Mn 2+  concentration. The thermal stability of the duplex in the presence of Cd 2+ , Ni 2+ , and Zn 2+  rises up to an ion concentration of about 10 −4  M and further falls. In contrast to these ions, the addition of Cu 2+  to the solution leads to a sharp decline in  Т  m  of the duplex at a concentration above 10 −5  M. The observed difference in the influence of metal ions on the poly(I:C) thermal stability is explained by the different affinity of these ions to both the phosphate groups and the nitrogenous bases of the polynucleotides. Based on the analysis of the  T  m  concentration dependence, the dominant binding of each ion to the specific polynucleotide components was proposed.

The Effect of Divalent Metal Ions on the Temperature Stability of Poly(I:C) Duplex