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Background   There is a shortage of chemical reagents for severe acute respiratory syndrome coronavirus 2 ( SARS-CoV-2 ) diagnosis and a surge of  SARS-CoV-2  cases, especially in limited-resource settings. Therefore, the combination of an optimal assay kit is necessary.Methods   We compared the ability to screen  SARS-CoV-2  among three primer-probe sets in two different master mixes, Invitrogen™ SuperScript™ III One-Step RT-PCR and LightCycler Multiplex RNA Virus Master.Results   The assay with TIB-Molbiol, IDT, and Phu Sa sets for LightCycler Multiplex RNA Virus Master or Invitrogen™ SuperScript™ III One-Step RT-PCR showed positive results from a single reaction of triplicate in the three days of 4.8 copies per reaction.  R  squared and amplification efficiency were 0.97 and ranged from 107 to 108%, respectively.Conclusions   Our findings indicated that TIB-Molbiol, IDT, and Phu Sa primer-probe sets could be beneficial for the laboratory screening of  SARS-CoV-2  by RT-qPCR assay of E gene. There is a need to consider the combination of these reagent sets as a new strategy to increase the testing capacity of screening programs for COVID-19.

biomed research international

Comparison of Primer-Probe Sets among Different Master Mixes for Laboratory Screening of Severe Acute Respiratory Syndrome Coronavirus 2 (<i>SARS-CoV-2</i>)

Comparison of Primer-Probe Sets among Different Master Mixes for Laboratory Screening of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)