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The aim of this study was to investigate genetic structures and expression of  bla  OXA-58  gene in five  Acinetobacter baumannii  clinical isolates recovered from two hospitals in southern Vietnam during 2012-2014.  A. baumannii  isolates were identified by automated microbiology systems and confirmed by PCR. All isolates were characterized as multidrug resistant by antimicrobial testing using the disk diffusion method. Four imipenem susceptible and one nonsusceptible isolates (MIC > 32  μ g·ml −1 ) were identified by E-test. PCR amplification of  bla  OXA-58  gene upstream and downstream sequences revealed the presence of IS Aba3  at both locations in one multidrug-resistant isolate. Semiquantitation of  bla  OXA-51  and  bla  OXA-58  gene expression was performed by the 2 - ΔΔ Ct  method. The  bla  OXA-51  gene expression of five isolates showed little difference, but the isolate bearing IS Aba3 - bla  OXA-58 -IS Aba3  exhibited significantly higher  bla  OXA-58  mRNA level. Higher  β -lactamases activity in periplasmic than cytoplasmic fraction was found in most isolates. The isolate overexpressing  bla  OXA-58  gene possessed very high periplasmic enzyme activity. In conclusion, the  A. baumannii  isolate bearing IS Aba3 - bla  OXA-58  gene exhibited high resistance to imipenem, corresponding to an overexpression of  bla  OXA-58  gene and very high periplasmic  β -lactamase activity.

biomed research international

Overexpression ofblaOXA-58Gene Driven by ISAba3Is Associated with Imipenem Resistance in a ClinicalAcinetobacter baumanniiIsolate from Vietnam