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Background   External quality assessment (EQA) provides evidence of reliable, accurate, and precise results for customers using the diagnostic test for  Toxocara canis. Objective . To establish a procedure for producing standard  Toxocara canis  serum samples for serodiagnostic testing in EQA.Methods   The collected serum samples to contain anti- Toxocara canis  serum samples for serodiagnostic testing in EQA.  F -test and a  T -test were applied to evaluate their homogeneity and stability.Results   Among eleven samples positive by ELISA, ten of them were confirmed via Western blotting by positive reaction with 5 specific  Toxocara canis  serum samples for serodiagnostic testing in EQA.  Toxocara canis  serum samples for serodiagnostic testing in EQA.  p  > 0.05). Samples produced by both methods were stable for 7 days at 30°C ( p  > 0.05). Samples produced by both methods were stable for 7 days at 30°C (    Conclusion   Specific serodiagnosis samples of anti- Toxocara canis  antibodies for EQA could be produced that possessed homogeneity and stability lasting for 3 months and 6 months by the freeze-drying and freezing methods, respectively. At 30°C, the samples produced by both methods were stable for 7 days, suitable for delivery to remote laboratories. Toxocara canis  serum samples for serodiagnostic testing in EQA.

journal of parasitology research

Establishment of an Experimental Procedure for Preparing Trial Serum Samples for the Specific Serodiagnosis of <i>Toxocara canis</i> for External Quality Assessment Schemes

Establishment of an Experimental Procedure for Preparing Trial Serum Samples for the Specific Serodiagnosis of Toxocara canis for External Quality Assessment Schemes