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Metallo-beta-lactamase-producing  Acinetobacter  spp. is a major challenge for therapeutic treatment of nosocomial infections. This study is aimed at determining the prevalence of MBL-producing  Acinetobacter  spp. among 87 clinical isolates of  Acinetobacter  spp. from the Korle-Bu Teaching Hospital, Accra, between August 2014 and July 2015.  Acinetobacter  spp. was identified by standard bacteriological method, and resistance to different antibiotics was assessed with the Kirby–Bauer disc diffusion method. Meropenem-resistant  Acinetobacter  isolates were screened for enzyme activity using the modified Hodge test (MHT) and combined disc test (CDT). Additionally, multiplex PCR was used to determine MBL genes presence ( bla VIM ,  bla IMP, and  bla NDM). All  Acinetobacter  isolates showed high resistance to cefotaxime (90.8%), ceftazidime (75.9%), cotrimoxazole (70.1%), ciprofloxacin (64.4%), gentamicin (72.4%), levofloxacin (67.8%), and meropenem (59.8%). A total of 54 (62.1%) of  Acinetobacter  isolates were multidrug-resistant. Out of 52 (59.8%) meropenem-resistant  Acinetobacter , 3 (5.8%) were carbapenemase producers by MHT, whilst, 23 (44.2%) were CDT positive. There was no significant difference between the resistance pattern of amikacin, ceftazidime, cotrimoxazole, ciprofloxacin, and meropenem amongst CDT-positive and CDT-negative isolates ( p  > 0.05). A total of 7/87 (8.1%) CDT-positive  Acinetobacter  isolates harboured  bla NDM; of these, 4 (57.1%) were from wound swabs, urine ( n  = 2) (28.6%), and ear swab ( n  = 1) (14.3%). The study revealed that less than 9% of  Acinetobacter  spp. contained  bla NDM encoding genes. Strict antibiotics usage plan and infection control measures are required to prevent the spread of these resistance genes.

Metallo-Beta-Lactamase-Producing Acinetobacter spp. from Clinical Isolates at a Tertiary Care Hospital in Ghana