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Crude methanol extracts of fruits, seeds, and bark of  Zanthoxylum armatum  were investigated in vitro for antimicrobial activities against 9 different bacterial strains:  Bacillus subtilis ,  Enterococcus faecalis ,  Proteus vulgaris ,  Pseudomonas aeruginosa ,  Salmonella typhi ,  Shigella dysenteriae ,  Staphylococcus aureus , methicillin-resistant  Staphylococcus aureus  (MRSA), and  Staphylococcus epidermidis  using agar well diffusion method, and the MBC values were determined. Only 5 bacteria, i.e.,  Bacillus subtilis, Enterococcus faecalis,  MRSA,  Staphylococcus aureus , and  Staphylococcus epidermidis  exhibited antibacterial properties against the different extracts. The fruit and seed extracts showed activities against 5 bacteria, while the bark extract was active against 2 bacteria only (MRSA and  Staphylococcus aureus ).  Staphylococcus aureus  was found to be more susceptible for all the extracts compared to other strains. The maximum ZOI of 20.72 mm was produced by fruits (wild) and 18.10 mm (cultivated) against  Staphylococcus aureus.  Likewise, the least ZOI of 11.73 mm was produced by seeds (wild) and 11.29 mm (cultivated) against  Escherichia faecalis.  Similarly, the lowest MBC value of 0.78 mg/mL was obtained for fruit extracts against MRSA, 1.56 mg/mL for fruits, seeds, and bark extracts against  Bacillus subtilis , MRSA, and  Staphylococcus aureus , and highest value of 50 mg/mL for fruits and seeds extracts against  S. epidermidis.  The fruits, seeds, and bark extracts of  Z. armatum  exhibited remarkable antibacterial properties against different pathogenic bacteria causing several diseases, which suggests the potential use of this plant for treating different bacterial diseases such as skin infection, urinary tract infection, dental problems, diarrhea, and dysentery.

In Vitro Antibacterial Activities of Methanolic Extracts of Fruits, Seeds, and Bark of Zanthoxylum armatum DC