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Magnetic Fe 3 O 4  nanoparticles were synthesized from maize leaves and plantain peels extract mediators. Particles were characterized, and the inhibitory effects were studied on HeLa cells in vitro using cyclic voltammetry (CV). Voltammograms from the CV show that Fe 3 O 4  NPs interaction with HeLa cells affected their electrochemical behavior. The nanoparticles formed with higher Fe 3+ /Fe 2+  molar ratio (2.8 : 1) resulted in smaller crystallite sizes compared to those formed with lower Fe 3+ /Fe 2+  molar ratio (1.4 : 1). The particles with the smallest crystallite size showed higher anodic peak currents, whereas the larger crystallite sizes resulted in lower anodic peak currents. The peak currents relate to cell inhibition and are confirmed by the half-maximum inhibitory concentration (IC 50 ). The findings show that the particles have a different inhibitory mechanism on HeLa cells ion transfer and are promising to be further exploited for cancer treatment.

Characterization and Inhibitory Effects of Magnetic Iron Oxide Nanoparticles Synthesized from Plant Extracts on HeLa Cells