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Oral administration of an aqueous extract of the aerial parts of  Bromelia karatas  to STZ-NA rats showed a significant hypoglycemic effect in a chronic trial lasting 42 days. Chromatographic profiles of the active extract (WE) and an organic extract (OE) of  B. karatas  were obtained by high-performance liquid chromatography (HPLC) and used to identify their major components. Isolation and identification of the compounds present in the extracts were accomplished by means of various conventional chromatographic and spectroscopic techniques. This process led to the identification of  β -sitosterol-3-O- β -D-glucopyranoside ( 1 ) and  ρ -coumaric acid ( 3 ) as the major compounds present in the extracts. During the isolation of  1  and  3 , seven additional metabolites not previously reported for the plant were obtained, namely, cirsiliol 4′-O- β -D-glucopyranoside ( 2 ), stigmasterol ( 4 ),  β -sitosterol ( 5 ), 1-O-feruloyl-3-O- ρ -coumaroylglycerol ( 6 ),  β -D-(1-O-acetyl-3,6-O-trans-diferuloyl) fructofuranosyl- α -D-2′,4′,6′-O-triacetylglucopyranoside ( 7 ), 1-O-p-coumaroyl-3-O-caffeoylglycerol ( 8 ), and 2-propyl- β -glucopyranoside ( 9 ).

Phytochemical Composition and Chronic Hypoglycemic Effect of Bromelia karatas on STZ-NA-Induced Diabetic Rats