English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Cannabis sativa  L. has been utilized for a long time as a traditional herbal medicine in Korea. Dry fruits, achenes, each containing a single seed of  Cannabis , are currently prescribed as  Ma In  (Cannabis Semen), a laxative. As each achene is enclosed by a bract, in which tetrahydrocannabinol (THC), the main psychological active compound in  Cannabis  is synthesized; achene is easily contaminated by THC from bract remnants. Therefore, it is safer to harvest achenes from  Cannabis  with a low THC content. Seeds of hemp, a low THC  Cannabis , were recently classified as possible sources of new pharmacologically active compounds. Thus, a proper method to select appropriate  Cannabis  plants with low THC among cultivars in South Korea for medicinal purpose is necessary. As a result of cross-selection,  Cannabis  L. cultivar “Cheungsam” (CH) with the lowest THC content among cultivars cultivated in South Korea has been developed. In this study, we developed two DNA markers to reliably discriminate CH from other local cultivars with higher THC contents. We developed primer sets CHF3/CHR2 to amplify the 642 bp DNA marker of CH based on differences in the nucleotide sequences of the  THCA synthase  gene, which encodes a key enzyme in THC synthesis. We then developed a CHF1/CHR3 primer set to amplify the 401 bp DNA marker of CH based on the differences in both the content of very long chain fatty acids (VLCFs) and the sequence of the putative  3-ketoacyl-CoA synthase  ( KCS ) gene encoding enzymes synthesizing VLCFs among local cultivars.

evidence-based complementary and alternative medicine

DNA Markers to Discriminate Cannabis sativa L. ‘Cheungsam’ with Low Tetrahydrocannabinol (THC) Content from Other South Korea Cultivars Based on the Nucleotide Sequences of Tetrahydrocannabinolic Acid Synthase and Putative 3-Ketoacyl-CoA Synthase Genes