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The quantitative analysis of multicomponents by the single-marker (QAMS) method was established and the relationship between  F  value (the ratio of the sum of the contents of emodin-8-O- β -D-glucopyranoside and physcion-8-O- β -D-glucopyranoside to the sum of the contents of emodin and physcion) and the steaming time was found to identify and differentiate  Polygonum multiflorum  Radix and its processed product. Emodin was considered as the control substance, and the correction factors of physcion, emodin-8-O- β -D-glucopyranoside, and physcion-8-O- β -D-glucopyranoside were computed. In addition, the contents of the four components were determined. When the  F  value is greater than or equal to 1.0, the sample was identified as  Polygonum multiflorum  Radix, and if the  F  value was between 0.6 and 1.0, the sample of  Polygoni multiflori  Radix Preaparata was processed incompletely. The  F  value of the qualified Radix  Polygonum multiflorum  should be no more than 0.6. However, the influence of different sample injection volumes and the chromatographic columns and instruments used on the durability of the correction factors and RSD ≤3% hindered accurate identification; therefore, a QAMS method using an external standard value with methodological verification was developed. We redefined the “ Polygonum multiflorum  rules.” The method using “ Polygonum multiflorum  rules” revised after optimization of the determination results was used, as it was accurate and led to convenient operation and low inspection costs, and moreover, the method could differentiate  Polygoni multiflori  Radix Preaparata and  Polygonum multiflorum  Radix medicinal samples and precisely identify samples that were different from the completely processed product  Polygoni multiflori  Radix Preaparata.

journal of analytical methods in chemistry

Identification and Differentiation of <i>Polygonum multiflorum</i> Radix and <i>Polygoni multiflori</i> Radix Preaparata through the Quantitative Analysis of Multicomponents by the Single-Marker Method

Identification and Differentiation of Polygonum multiflorum Radix and Polygoni multiflori Radix Preaparata through the Quantitative Analysis of Multicomponents by the Single-Marker Method