English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The role of gamma delta ( γδ ) T cells in human cytomegalovirus (HCMV) immune surveillance has been the focus of research interest for years. Recent reports have shown a substantial clonal proliferation of  γδ  T cells in response to HCMV, shedding light on the adaptive immune response of  γδ  T cells. Nevertheless, most efforts have focused on V δ 2 neg  γδ  T cell subset while less attention has been given to investigate other less common  γδ  T cell subsets. In this regard, a distinct subpopulation of  γδ  T cells that expresses the CD8 coreceptor (CD8 +  γδ  T cells) has not been thoroughly explored. Whether it is implicated in HCMV response and its ability to generate adaptive response has not been thoroughly investigated. In this study, we combined flow cytometry and immune sequencing of the TCR  γ -chain ( TRG ) to analyze in-depth bone marrow (BM) graft  γδ  T cells from CMV seropositive (CMV+) and CMV seronegative (CMV-) donors. We showed that the frequency of CD8 +  γδ  T cells was significantly higher in CMV+ grafts compared to CMV- grafts ( P  < 0.001). Further characterization revealed that CD8 +  γδ  T cells from CMV+ grafts express V γ 9 −  and preferentially differentiated from a naive to terminal effector memory phenotype (CD27 low/- CD45RO − ). In line with these findings,  TRG  immune sequencing revealed clonal focusing and reduced usage of the V γ 9/JP gene segment in a CMV+ graft. Furthermore, CD8 +  γδ  T cells showed an enhanced response to TCR/CD3 and cytokine stimulation in contrast to CD8 −  γδ  T cells. We conclude that  γδ  T cells in BM grafts are reshaped by donor CMV serostatus and highlight the potential adaptive role of CD8 +  γδ  T cells in HCMV immune response.

CD8+γδ T Cells Are More Frequent in CMV Seropositive Bone Marrow Grafts and Display Phenotype of an Adaptive Immune Response

CD8⁺γδ T Cells Are More Frequent in CMV Seropositive Bone Marrow Grafts and Display Phenotype of an Adaptive Immune Response