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The aim of this study was to obtain and characterise the long-chain alcohols present in policosanol derived from waste from the production of carminic acid, a natural colouring agent widely used in the food industry. The effectiveness of different methods designed for extraction of policosanol from waxy waste was investigated and its content and composition was determined. Triacontanol was the main component in policosanol produced by chemical processes, and it yields up to 13% by alkaline hydrolysis in water and chloroform extraction. Regarding enzymatic transesterification, policosanol was obtained using lipase Candida antarctica recombinant in Aspergillus niger (CAL-Bn) in a reaction medium with toluene. To improve the reaction, different acyl receptors, propanol, butanol, and isopropanol, were tested and molecular sieves were employed to maintain an anhydrous reaction medium. In this case, the policosanol was made up of other long-chain alcohols, but triacontanol was obtained in yields of up to 19% using isopropanol as an acyl receptor. Triacontanol has a great commercial value due to its effect as a promoter of plant growth, and these results contribute to the use and application of this agroindustrial waste in obtaining value-added products.

Cochineal Waxy Residues as Source of Policosanol: Chemical Hydrolysis and Enzymatic Transesterification