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In this study, comparative experiments were carried out to investigate the positional selectivity of  Candida  sp. 99–125 lipase in preparing 1,3-diolein by using medium engineering strategy. The results indicated that the diolein yield was markedly enhanced from 56.5% to 86.7% with increasing log⁡ P  values of the solvents, while the selectivity of the examined lipase for the  sn-1  over the  sn-2  hydroxyl of glycerol was decreased, thus leading to a reduced 1,3-diolein to 1,2-diolein ratio. To evaluate the possibility of industrial enzymatic production of 1,3-diolein, larger-scale experiments were assessed. After being used repeatedly for eight batches, the diolein content reached 95.1%, while the 1,3-diolein to 1,2-diolein ratio was 7:1 following purification. Results of the  kg  level experiments significantly demonstrated the practicability of the enzymatic process and the efficiency of the purification strategy for the product.

Evaluation of the Candida sp. 99-125 Lipase Positional Selectivity for 1,3-Diolein Synthesis