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Dendranthema indicum  var.  aromaticum  is a new species with strong fragrance and is used as a herbal medicine by Chinese folks. The abundant flavonoids play important roles in its pharmacological activities. In this study, an ultrasound-assisted method was used to extract total flavonoids (TF) from  D. indicum  var.  aromaticum  by response surface methodology. A quadratic model was developed to optimize the extraction conditions, whose accuracy was verified by statistic analysis. Ethanol and acetic acid at the volume ratio of 70% : 2% were selected as the extract solvent. The optimized extraction conditions were as follows: extraction time, 40 min; solid/liquid ratio, 1 : 23 g/mL; and temperature, 60°C. This is the first report of an efficient and easy-operating method for TF extraction from  D. indicum  var.  aromaticum . Besides, this study provides reference for future pharmacological research on  D. indicum  var.  aromaticum  and extraction of bioactive components from other herbs.

journal of analytical methods in chemistry

Optimization of Ultrasound-Assisted Extraction of Total Flavonoids from <i>Dendranthema indicum</i> var<i>. aromaticum</i> by Response Surface Methodology

Optimization of Ultrasound-Assisted Extraction of Total Flavonoids from Dendranthema indicum var. aromaticum by Response Surface Methodology