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Cellulases are a group of hydrolytic enzymes that break down cellulose to glucose units. These enzymes are used in the food, beverage, textile, pulp, and paper and the biofuel industries. The aim of this study was to isolate fungi from natural compost and produce cellulases in submerged fermentation (SmF). Initial selection was based on the ability of the fungi to grow on agar containing Avicel followed by cellulase activity determination in the form of endoglucanase and total cellulase activity. Ten fungal isolates obtained from the screening process showed good endoglucanase activity on carboxymethyl cellulose-Congo Red agar plates. Six of the fungal isolates were selected based on high total cellulase activity and identified as belonging to the genera  Trichoderma  and  Aspergillus . In SmF of synthetic media with an initial pH of 6.5 at 30°C  Trichoderma longibrachiatum  LMLSAUL 14-1 produced total cellulase activity of 8 FPU/mL and endoglucanase activity of 23 U/mL whilst  Trichoderma harzianum  LMLBP07 13-5 produced 6 FPU/mL and endoglucanase activity of 16 U/mL. The produced levels of both cellulases and endoglucanase by  Trichoderma  species were higher than the levels for the  Aspergillus fumigatus  strains.  Aspergillus fumigatus  LMLPS 13-4 produced higher  β -glucosidase 38 U/mL activity than  Trichoderma  species.

Isolation of Cellulose Degrading Fungi from Decaying Banana Pseudostem andStrelitzia alba