English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Objective   To investigate the role of microRNA-130b in 1,25(OH)2D3 mediated improvement of renal fibrosis via transforming growth factor-beta 1 in a rat model of diabetic nephropathy (DN).Methods   DN was induced in 30 rats by intraperitoneal injection of streptozotocin. These rats were randomly allocated to the DN group, TGF- β 1 overexpression group ( in situ  injection of TGF- β 1 lentivirus to kidney tissues), and TGF- β 1 siRNA group ( in situ  injection of TGF- β 1 siRNA lentivirus to kidney tissues). Rats with different expression levels of TGF- β 1 were administered 1,25(OH)2D3 (0.03  μ g/kg/d) or peanut oil as control. DN rats were treated only with peanut oil. All rats were randomly divided into five groups ( n  = 6 per group): TGF- β 1 overexpression + oil, TGF- β 1 overexpression + 1,25(OH)2D3, TGF- β 1 siRNA + oil, TGF- β 1 siRNA + 1,25(OH)2D3, and DN + oil groups. After 37 days, kidney samples were collected and the expression of TGF- β 1 and miR-130b was determined by real-time PCR, western blotting, and immunohistochemistry. Hematoxylin and eosin staining and Masson staining were used to evaluate kidney morphological and fibrogenic changes. Differences were determined using ANOVA and Student's  t -test.Results   RT-PCR, western blotting, and immunohistochemistry revealed that interference of TGF- β 1 significantly decreased mRNA and protein levels of TGF- β 1 in renal tissues of DN rats compared to those in renal tissues of rats overexpressing TGF- β 1 ( p  < 0.05). Histological analysis showed that upregulated TGF- β 1 led to disorganized kidney structure and severe kidney fibrosis. The expression of miR-130b was significantly lowered upon lentivirus-mediated overexpression of TGF- β 1 than upon downregulation of TGF- β 1 ( p  < 0.05). Treatment with 1,25(OH)2D3 led to a significant reduction of TGF- β 1 at the mRNA and protein levels (both  p  < 0.05), improvement of renal structure and fibrosis, and an increase in miR-130b expression ( p  < 0.05).Conclusion   TGF- β 1 can decrease the expression of miR-130b in kidney tissues of DN rats. Moreover, miR-130b may be involved in the protective effect of 1,25(OH)2D3 on renal fibrosis via TGF- β 1.

Regulatory Effect of 1,25(OH)2D3 on TGF-β1 and miR-130b Expression in Streptozotocin-Induced Diabetic Nephropathy in Rats