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Fascioliasis—a disease caused by  Fasciola  spp. (Platyhelminthes: Trematoda: Digenea)—is considered as the most important helminthic infection of bovine, sheep, and buffalo in Vietnam. The aim of this study is to detect the genotype of  Fasciola  spp. isolated from bovine and buffalo in the Nghe An province, central Vietnam, using PCR-RFLP and sequence analysis of the first nuclear ribosomal internal transcribed spacer (ITS1). Adult  Fasciola  spp. were isolated from bile ducts of bovine and buffalo in Nghe An province, Vietnam. Overall, 96 adult flukes from livers of slaughtered animals were collected from abattoirs of different areas. They included 7 samples from infected bovine and 89 samples from infected buffalo. 96/96 samples were identified as  Fasciola  species by ITS1 of rDNA. In this study, a PCR-RFLP method was used to distinguish between  F. hepatica  and  F. gigantica  in ITS1 of rDNA (680 bp) with  Rsa I restriction enzyme. RFLP pattern with  Rsa I produced a consistent pattern of 360, 100, and 60 bp fragments in  F. hepatica , whereas  F. gigantica  worms had a profile of 360, 170, and 60 bp in size, respectively. The results showed that using PCR-RFLP based on the first internal transcribed spacers (ITS1) of the ribosomal RNA revealed that 93 out of 96 isolates were of  Fasciola gigantica  type, whereas three isolates presented an intermediate  Fasciola . In the present study,  F. gigantica  and intermediate form were coexisting in bovine and buffalo in the Nghe An province of central Vietnam, whereas  F. hepatica  was not detected.

journal of parasitology research

Identification of<i> Fasciola</i> Species Isolates from Nghe An Province, Vietnam, Based on ITS1 Sequence of Ribosomal DNA Using a Simple PCR-RFLP Method

Identification of Fasciola Species Isolates from Nghe An Province, Vietnam, Based on ITS1 Sequence of Ribosomal DNA Using a Simple PCR-RFLP Method