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Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains,  Lactococcus lactis  SLT10,  Lactobacillus plantarum  C7, and  Leuconostoc mesenteroides  B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane. EPS recovery by ultrafiltration was higher than ethanol precipitation for  Lactococcus lactis  SLT10 and  Lactobacillus plantarum  C7. However, it was similar with both methods for  Leuconostoc mesenteroides  B3. The monomer composition of the EPS fractions revealed differences in structures and molar ratios between the two studied methods. EPS isolated from  Lactococcus lactis  SLT10 are composed of glucose and mannose for EPS-ETOH against glucose, mannose, and rhamnose for EPS-UF. EPS extracted from  Lactobacillus plantarum  C7 and  Leuconostoc mesenteroides  B3 showed similar composition (glucose and mannose) but different molar ratios. The molecular weights of the different EPS fractions ranged from 11.6±1.83 to 62.4±2.94 kDa. Molecular weights of EPS-ETOH fractions were higher than those of EPS-UF fractions. Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.

biomed research international

Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria