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Here we report functional characterization of the  Streptomyces coelicolor  M145 gene  SCO1678,  which encodes a GntR-like regulator of the FadR subfamily. Bioinformatic analysis suggested that  SCO1678  is part of putative operon  (gnt)  involved in gluconate metabolism. Combining the results of  SCO1678  knockout, transcriptional analysis of  gnt  operon, and Sco1678 protein-DNA electromobility shift assays, we established that Sco1678 protein controls the gluconate operon. It does so via repression of its transcription from a single promoter located between genes  SCO1678  and  SCO1679 . The knockout also influenced, in a medium-dependent manner, the production of secondary metabolites by  S. coelicolor . In comparison to the wild type, on gluconate-containing minimal medium, the  SCO1678  mutant produced much less actinorhodin and accumulated a yellow-colored pigment, likely to be the cryptic polyketide coelimycin. Possible links between gluconate metabolism and antibiotic production are discussed.

Role of GntR Family Regulatory GeneSCO1678in Gluconate Metabolism inStreptomyces coelicolorM145