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Here we report, for the first time, the differential cellular distribution of two melanopsins (Opn4m1 and Opn4m2) and the effects of GR agonist, dexamethasone, on the expression of these opsins and clock genes, in the photosensitive  D. rerio  ZEM-2S embryonic cells. Immunopositive labeling for Opn4m1 was detected in the cell membrane whereas Opn4m2 labeling shows nuclear localization, which did not change in response to light.  opn4m1 ,  opn4m2 ,  gr ,  per1b,  and  cry1b  presented an oscillatory profile of expression in LD condition. In both DD and LD condition, dexamethasone (DEX) treatment shifted the peak expression of  per1b  and  cry1b  transcripts to ZT16, which corresponds to the highest  opn4m1  expression. Interestingly, DEX promoted an increase of  per1b  expression when applied in LD condition but a decrease when the cells were kept under DD condition. Although DEX effects are divergent with different light conditions, the response resulted in clock synchronization in all cases. Taken together, these data demonstrate that  D. rerio  ZEM-2S cells possess a photosensitive system due to melanopsin expression which results in an oscillatory profile of clock genes in response to LD cycle. Moreover, we provide evidence that glucocorticoid acts as a circadian regulator of  D. rerio  peripheral clocks.

Dexamethasone Modulates Nonvisual Opsins, Glucocorticoid Receptor, and Clock Genes inDanio rerioZEM-2S Cells