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The concentration of diterpenes barbatusin ( 1 ) and 3 β -hydroxy-3-deoxybarbatusin ( 2 ) in the extracts from leaves of  Plectranthus grandis, P. barbatus, P. ornatus,  and  P. amboinicus  was evaluated by HPLC-DAD analysis on a Luna C-18 column, using isocratic mixtures of water and acetonitrile as eluents. The regression equations were obtained with good linearity ( r  2  > 0.99) and limit of quantifications was higher than 0.1  µ g/mL. The precision (lower than 3.5%, within day) and accuracy (higher than 81.7% and lower than 107.6%) of the methods were adequate. Barbatusin ( 1 ) was detected in  P. grandis  (15.432 ± 2.28 mg/g) and  P. barbatus  (5.198 ± 3.45 mg/g) extracts, while compound  2  was detected in  P. grandis  (4.068 ± 3.34 mg/g),  P. barbatus  (0.654 ± 5.86 mg/g),  P. amboinicus  (0.160 ± 7.25 mg/g), and  P. ornatus  (0.763 ± 5.10 mg/g). The evaluated validation parameters were satisfactorily achieved, and the developed methodology represents a suitable tool for application in the quantification of barbatusin ( 1 ) and 3 β -hydroxy-3-deoxybarbatusin ( 2 ) in  Plectranthus  species.

Quantification of Barbatusin and 3β-Hydroxy-3-deoxybarbatusin in Plectranthus Species by HPLC-DAD