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A simple HPLC-UV method has been developed and validated for the quantification of ellagic acid (EA) in ethanol extracts of  Eugenia uniflora  L. (Myrtaceae) leaves. The ultrasound-assisted extraction (UAE) optimization was performed using a Box Behnken design (3 3 ) combined with response surface methodology to study the effects of the ethanol concentration (%, w/w), extraction time (minutes), and temperature (°C) on the EA concentration. The optimized results showed that the highest extraction yield of EA by UAE was 26.0  μ g mL −1  when using 44% (w/w) ethanol as the solvent, 22 minutes as the extraction time, and 59°C as the extraction temperature. The concentration of EA in relation to the predicted value was 93.7%  ±  0.4. UAE showed a strong potential for EA extraction.

evidence-based complementary and alternative medicine

Development and Validation of a HPLC-UV Method for the Evaluation of Ellagic Acid in Liquid Extracts of<i> Eugenia uniflora</i> L. (Myrtaceae) Leaves and Its Ultrasound-Assisted Extraction Optimization

Development and Validation of a HPLC-UV Method for the Evaluation of Ellagic Acid in Liquid Extracts of Eugenia uniflora L. (Myrtaceae) Leaves and Its Ultrasound-Assisted Extraction Optimization