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This study investigates the synergy of  Rhizobium phaseoli  and  Acidithiobacillus ferrooxidans  in the bioleaching process of copper. The results showed that additional  R. phaseoli  could increase leaching rate and cell number of  A. ferrooxidans . When the initial cell number ratio between  A. ferrooxidans  and  R. phaseoli  was 2 : 1,  A. ferrooxidans  attained the highest final cell number of approximately 2 × 10 8  cells/mL and the highest copper leaching rate of 29%, which is 7% higher than that in the group with  A. ferrooxidans  only.  R. phaseoli  may use metabolized polysaccharides from  A. ferrooxidans , and organic acids could chelate or precipitate harmful heavy metals to reduce their damage on  A. ferrooxidans  and promote its growth. Organic acids could also damage the mineral lattice to increase the leaching effect.

Synergy betweenRhizobium phaseoliandAcidithiobacillus ferrooxidansin the Bioleaching Process of Copper