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Progesterone (P4) and estradiol (E2) play a significant role in mammalian reproduction. Our study demonstrated that separated porcine cumulus cells (CCs) and/or granulosa cells (GCs) might proliferate  in vitro  during short-term, real-time primary culture. The GCs were analyzed according to gene expression of the progesterone receptor (nuclear form) ( pgr ), progesterone receptor membrane component 1 ( pgrmc1 ), and estrogen-related receptor beta 3 ( esrrb3 ) in relation to two housekeeping genes:  actb  and  pbgd . GCs were cultivated in medium with the E2. Both  pgr / actb  and  pgr / pbgd  revealed higher expression between 24 and 168 h of IVC of prolonged E2 treatment and at 48 h of IVC after acute E2 administration. The  pgrmc1 / actb  and  pgrmc1 / pbgd  displayed increased expression after prolonged E2 treatment between 24 and 120 h of IVC. The highest level of  esrrb3 / actb  at 120 and 144 h, as well as  esrrb3 / pbgd  at 120 h, in untreated controls as compared to the hormone-stimulated group, was observed. We suggest that E2 significantly influences the upregulation of  pgr ,  pgrmc1 , and  esrrb3  expression in porcine GCs during real-time cell proliferation. Since  esrrb3  expression is stimulated by E2 in both an acute and prolonged manner, estradiol may be recognized as a potential estrogen receptor agonist in GCs.

biomed research international

Influence of Estradiol-17beta on Progesterone and Estrogen Receptor mRNA Expression in Porcine Follicular Granulosa Cells during Short-Term,<i> In Vitro</i> Real-Time Cell Proliferation

Influence of Estradiol-17beta on Progesterone and Estrogen Receptor mRNA Expression in Porcine Follicular Granulosa Cells during Short-Term, In Vitro Real-Time Cell Proliferation