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Aim . The purpose of this study was to compare the in vitro efficacy of calcium hypochlorite (Ca[OCl] 2 ) and sodium hypochlorite (NaOCl) associated with sonic (Vibringe) irrigation system in root canals which were contaminated with  Enterococcus faecalis .  Material and Methods . The root canals of 84 single-rooted premolars were enlarged up to a file 40, autoclaved, inoculated with  Enterococcus faecalis , and incubated for 21 days. The samples were divided into 7 groups according to the irrigation protocol: G0: no treatment; G1: distilled water; G2: 2.5% NaOCl; G3: 2.5% Ca(OCl) 2 ; G4: distilled water with sonic activation; G5: 2.5% NaOCl with sonic activation; and G6: 2.5% Ca(OCl) 2  with sonic activation. Before and after decontamination procedures microbiological samples were collected and the colony-forming units were counted and the percentages of reduction were calculated.  Results . Distilled water with syringe irrigation and sonic activation groups demonstrated poor antibacterial effect on  Enterococcus faecalis  compared to other experimental groups ( p  < 0.05). There was no statistically significant difference between syringe and sonic irrigation systems with Ca(OCl) 2  and NaOCl.  Conclusion . The antimicrobial property of Ca(OCl) 2  has been investigated and compared with that of NaOCl. Both conventional syringe irrigation and sonic irrigation were found effective at removing  E. faecalis  from the root canal of extracted human teeth.

Antibacterial Efficacy of Calcium Hypochlorite with Vibringe Sonic Irrigation System onEnterococcus faecalis: An In Vitro Study