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Listeria monocytogenes  is a foodborne pathogen, and the detection and differentiation of this bacterium from the nonpathogenic  Listeria  species are of great importance to the food industry. Differentiation of  Listeria  species is very difficult, even with the sophisticated MALDI-TOF MS technique because of the close genetic relationship of the species and the usual gene transfer. The present paper emphasizes the difficulties of the differentiation through the standardized detection and confirmation according to ISO 11290-1:1996 and basic available  L. monocytogenes  detection methods and tests (such as API  Listeria  test, MALDI-TOF MS analysis, and  hly  gene PCR). With the increase of reports on the pathogenesis of atypical  Listeria  strains in humans, the significance of species level determination has become questionable, especially in food quality control, and the detection of pathogenic characteristics seems to be more relevant.

FoodborneListeria monocytogenes: A Real Challenge in Quality Control