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Fruits of  Ziziphus jujuba  Mill. (family: Rhamnaceae), known as Indian jujube or “Ber,” are of potential nutritional and medicinal value. The objectives of the present study were to estimate bioactive compound maslinic acid by HPTLC method and to evaluate  in vitro  antioxidant and hepatoprotective activity of eight cultivars of Indian jujube. Maslinic acid and the fruit pulp of various cultivars of Indian jujube, namely,  Gola ,  Sannur ,  Umaran ,  Mehrun , and  Chhuhara , exhibited significantly high antioxidant and hepatoprotective activity. HPTLC-densitometric method was developed for quantification of maslinic acid from fruits of Indian jujube cultivars. The trend of occurrence of maslinic acid in fruits pulp extracts was as follows:  Gola  >  Sannur  >  Umaran  >  Mehrun  >  Chhuhara  > Wild >  Kadaka  >  Apple . A significant correlation was shown by maslinic acid content and prevention of oxidative stress induced by CCl 4  in liver slice culture cell treated with extract. Maslinic acid along with its other phytoconstituents like phenols, flavonoids, and ascorbic acid may act as a possible therapeutic agent for preventing hepatotoxicity caused by oxidative stress generated due to the prooxidants like CCl 4 . This is the first report of fruit pulp extracts of  Z. jujube  cultivars in India and maslinic acid preventing CCl 4  induced damage in liver slice culture cell of mice.

evidence-based complementary and alternative medicine

Estimation of Bioactive Compound, Maslinic Acid by HPTLC, and Evaluation of Hepatoprotective Activity on Fruit Pulp of<i>Ziziphus jujuba</i>Mill. Cultivars in India

Estimation of Bioactive Compound, Maslinic Acid by HPTLC, and Evaluation of Hepatoprotective Activity on Fruit Pulp ofZiziphus jujubaMill. Cultivars in India