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To clone and express the  γ -polyglutamic acid ( γ -PGA) synthetase gene  pgs BCA in  Bacillus subtilis , a pWB980 plasmid was used to construct and transfect the recombinant expression vector pWB980- pgs BCA into  Bacillus subtilis  WB600.  PgsBCA  was expressed under the action of a P43 promoter in the pWB980 plasmid. Our results showed that the recombinant bacteria had the capacity to synthesize  γ -PGA. The expression product was secreted extracellularly into the fermentation broth, with a product yield of 1.74 g/L or higher.  γ -PGA samples from the fermentation broth were purified and characterized. Hydrolysates of  γ -PGA presented in single form, constituting simple glutamic acid only, which matched the characteristics of the infrared spectra of the  γ -PGA standard, and presented as multimolecular aggregates with a molecular weight within the range of 500–600 kDa. Expressing the  γ -PGA synthetase gene  pgsBCA  in  B. subtilis  system has potential industrial applications.

Cloning and Expression of theγ-Polyglutamic Acid Synthetase GenepgsBCA inBacillus subtilisWB600