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The paper focuses on the  in vitro  antimicrobial activity of  Lavandula angustifolia  Mill. (lavender) essential oil in combination with four commercial antimicrobial agents. Stock solutions of chloramphenicol, ciprofloxacin, nystatin, and fusidic acid were tested in combination with  L. angustifolia  essential oil. The antimicrobial activities of the combinations were investigated against the Gram-positive bacterial strain  Staphylococcus aureus  (ATCC 6538) and Gram-negative  Pseudomonas aeruginosa  (ATCC 27858) and  Candida albicans  (ATCC 10231) was selected to represent the yeasts. The antimicrobial effect was performed using the minimum inhibitory concentration (MIC) microdilution assay. Isobolograms were constructed for varying ratios. The most prominent interaction was noted when  L. angustifolia  essential oil was combined with chloramphenicol and tested against the pathogen  P. aeruginosa  (ΣFIC of 0.29).  Lavendula angustifolia  essential oil was shown in most cases to interact synergistically with conventional antimicrobials when combined in ratios where higher volumes of  L. angustifolia  essential oil were incorporated into the combination.

The In Vitro Antimicrobial Effects of Lavandula angustifolia Essential Oil in Combination with Conventional Antimicrobial Agents