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The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) was employed to acquire the infrared spectra of  Radix Bupleuri  and its unofficial varieties: the root of  Bupleurum smithii  Wolff and the root of  Bupleurum bicaule  Helm. The infrared spectra and spectra of Fourier self-deconvolution (FSD), discrete wavelet transform (DWT), and probability neural network (PNN) of these species were analyzed. By the method of FSD, there were conspicuous differences of the infrared absorption peak intensity of different types between  Radix Bupleuri  and its unofficial varieties. But it is hard to tell the differences between the root of  Bupleurum smithii  Wolff and the root of  Bupleurum bicaule . The differences could be shown more clearly when the DWT was used. The research result shows that by the DWT technology it is easier to identify  Radix Bupleuri  from its unofficial varieties the root of  Bupleurum smithii  Wolff and the root of  Bupleurum bicaule .

international journal of analytical chemistry

Study on the Identification of<i>Radix Bupleuri</i>from Its Unofficial Varieties Based on Discrete Wavelet Transformation Feature Extraction of ATR-FTIR Spectroscopy Combined with Probability Neural Network

Study on the Identification ofRadix Bupleurifrom Its Unofficial Varieties Based on Discrete Wavelet Transformation Feature Extraction of ATR-FTIR Spectroscopy Combined with Probability Neural Network