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The in vitro response of human fibroblast cell line HFL1 and human osteoblast cell line hFOB 1.19 on nanostructured titanium with different grain sizes has been compared in the present study. Used samples of titanium produced by equal channel angular (ECA) pressing have grain sizes of 160 nm, 280 nm, and 2400 nm with cross- and longitudinal sections. Similar cellular behaviour was observed on all studied biomaterials. There were significant differences related to the initial phase of attachment, but not in proliferation. Furthermore, the results indicate that osteoblasts grow best on material with grain size of 160 nm with a longitudinal section in comparison with other examined materials. Therefore, this material could be recommended for further evaluation with respect to osseointegration in vivo

Comparison of Fibroblast and Osteoblast Response to Cultivation on Titanium Implants with Different Grain Sizes